Ecto-5′nucleotidase (CD73) May Predict Occurrence and Grade of GVHD in Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation

BACKGROUND: Graft versus host disease (GVHD) represents a main cause of post-transplant morbidity and mortality. Ectonucleotidases are one major components purinergic signaling which is the important mediator pathways regulating cellular functions. CD73 most significant member ectonucleotidases. AIM: The aim study was to assess role in development/severity GVHD among patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) SUBJECT AND METHODS: This prospective conducted on 30 adult eligible for HSCT their donors period 2 years from January 2015 until 2017. Assessment positive cells through flow cytometry peripheral blood samples during assessment before receiving G-CSF recipients conditioning at day −7 once occurs (within 12 months) or end follow-up done. RESULTS: level significantly higher pre/post-transplantation (58.24 ± 19.68, 65.78 19.03 respectively) than (29.08 ±14.14) (p = 0.022), there negative correlation between pre-transplant occurrence chronic (cGVHD), 0.004) its grade 0.0496). Ectonucleotidase expression good predictor cGVHD with sensitivity 100% Specificity 65% cut off value ≤61.07%. independently predicting cGVHD. CONCLUSION: CD 73 may represent promising, clinically applicable tool HSCT.